This course is given at Scilifelab Solna, Rooms Air & Fire, ground floor to the left from the main entrance.
Lecture slides will be provided as links as they get completed.
Monday 21st of May
09.00-09.15 Introduction (Åsa Björklund)
09.15-10.00 scRNAseq methodologies and ESCG platform (Henrik Gezelius)
10.30-11.15 Introduction to read alignment pipelines and gene expression estimates (Johan Reimegård)
11.15-12.00 scRNAseq quality control (Åsa Björklund)
12.00-13.00 Lunch
13.00-17.00 Practical Exercises(Stefania Giaccomello, Åsa Björklund, Estelle Proux-Vera, Nikolay Oskolkov)
18.00- Course dinner at Shanti - Touch of Bengal
Tuesday 22nd of May
09.00-09.45 Dimensionality reduction (Per Unneberg)
10.15-11.00 Data normalization (Nikolay Oskolkov)
11.00-12.00 Differential gene expression (Olga Dethlefsen)
12.00-13.00 Lunch
13.00-14.00 Clustering techniques and scRNAseq toolkits (Åsa Björklund)
14.00-17.00 Practical exercises(Åsa Björklund, Olga Dethlefsen, Nikolay Oskolkov)
Wednesday 23rd of May
09.00-09.45 Pseudotime and trajectory analysis (Stefania Giaccomello)
10.15-11.00 RNA Velocity (Gioele La Manno)
11.00-12.00 Spatial mapping of single cells (Stefania Giaccomello)
13.00-16.00 Practical exercises (Åsa Björklund, Stefania Giacomello, Anna Johansson)
16.00-17.00 Summary of the week (Åsa Björklund)
Each day we will have cofee around 10.00 and at 15.00 during the exercises.
All lunches will be at Könings Restaurant (SMI building across the road).